黄连素对慢性阻塞性肺疾病大鼠气道炎症的影响

摘要

目的：探讨不同剂量黄连素对慢性阻塞性肺疾病(COPD)模型大鼠气道炎症的影响。方法50只清洁级雄性Wistar大鼠随机分为生理盐水对照组、COPD模型组和低剂量(25 mg/kg)、中剂量(50 mg/kg)、高剂量(100 mg/kg)黄连素组，每组各10只。通过气管注射内毒素加熏香烟方法建立大鼠COPD模型。采集左肺支气管肺泡灌洗液，利用瑞氏-吉姆萨染色法行中性粒细胞(PMN)、肺泡巨噬细胞(AM)计数；ELISA法检测IL-8、IL-10、IL-17含量。结果 COPD模型组大鼠支气管肺泡灌洗液中的PMN、AM计数及IL-8、IL-17含量显著高于生理盐水对照组(P<0.01)；IL-10含量显著低于生理盐水对照组(P<0.01)。而不同剂量黄连素干预后PMN、AM计数，IL-8、IL-17水平有不同程度下降(P<0.01)，IL-10水平有不同程度升高(P<0.01)，且黄连素剂量越高，此效应越明显。结论不同剂量黄连素均可抑制COPD模型大鼠的气道炎症，其机制可能与黄连素下调炎症因子IL-8、IL-17水平，增强抗炎因子IL-10水平这一途径有关，且此效应与黄连素剂量呈正相关。%Objective To investigate the effects of Berberine on the airway inflammation of rats with chronic obstructive pulmonary disease. Methods Fifty male and healthy Wistar rats were randomly divided into five groups, with 10 rats in each group, including NS control group, COPD group and 25, 50, 100 mg/kg Berberine group. Chronic obstructive pul-monary disease rats models were established by passive cigarette smoking plus intratracheal instillation of lipopolysac-charide. Bronchoalveolar lavage fluid (BALF) of left lung sample were collected. Polymorphonuclear (PMN) and alveolar macrophage (AM) counts in BALF were measured with Wright-Giemsa staining method. Interleukin-8 (IL-8), inter-leukin-10 (IL-10), interleukin-17 (IL-17) were detected with enzyme linked immunosorbent assay. Results The levels of PMN, AM counts and IL-8, IL-17 levels were significantly increased in BALF of COPD model group compared with saline control group (P<0.01), however, the level of IL-10 was just on the contrary (P< 0.01). After intervention with different doses of Berberine, compared with COPD model group, the levels of PMN, AM counts and IL-8, IL-17 levels were significantly decreased in BALF (P< 0.01), however, the level of IL-10 was increased to varying degrees (P<0.01), and the higher the dose of Berberine, this effects were more obvious. Conclusion Different doses of Berberine can inhibit the airway inflammation of COPD rat model. The mechanism is associated with Berberine lowered levels of inflammatory cytokine IL-8, IL-17 and enhance the level of anti-inflammatory cytokine IL-10, and this positive effect of Berberine is related to its dose.