目的：观察胃癌组织中微小RNA-181a(miR-181a)与其靶基因共济失调-毛细血管扩张突变基因(ATM)的表达与异常情况，初步研究其意义。方法收集2009年4月~2011年12月广州市第一人民医院外科手术切除的胃癌组织标本9例，同时选取其癌旁非癌组织标本作为对照，分析胃癌组织中miR-181a与ATM蛋白表达的相关性。提取其蛋白质及总RNA，采用实时荧光定量PCR(qRT-PCR)检测标本的miR-181a，Western blot检测ATM蛋白。比较胃癌组织及癌旁非癌组织中miR-181a、ATM蛋白表达量。结果 miR-181a在胃癌组织中的表达量为(1.981±1.800)，miR-181a在邻近非癌组织中的表达量为(0.394±0.093)；灰度值检测：癌组织ATM蛋白为(0.2539±0.0046)；非癌组织为(0.5525±0.0660)，癌及非癌组织中miR-181a、ATM蛋白表达量比较，差异均有高度统计学意义(P<0.01)；miR-181a与ATM蛋白表达呈负相关(r=-0.539，P<0.01)。结论 miR-181a在胃癌组织中表达明显增高，ATM蛋白表达明显下降；究其原因可能是通过基因沉默机制miR-181a降低ATM蛋白表达量，从而在胃癌的发生及发展中起促进作用。%Objective To investigate the expression and significance of microRNA (miRNA)-181a (miR-181a) and its target gene ATM in gastric carcinoma tissues. Methods Tissues of gastric carcinoma and adjacent non-tumorous were collected respectively from 9 patients given surgical operation in Guangzhou First People's Hospital from April 2009 to December 2011. The total RNA and protein were extracted routinely, the miR-181a was detected by Real-time quanti-tative PCR, ATM protein was detected by Western blot. The expression of miR-181a and ATM protein between gastric carcinoma and adjacent non-tumorous were compared, and the correlation between miR-181a and ATM protein levels in gastric carcinoma was analyzed. Results In gastric carcinoma tissues, the expression of miR-181a and ATM protein were (1.981±1.800), (0.2539±0.0046) respectively, whereas in the adjacent non-tumorous tissues, the expression of miR-181a and ATM protein were (0.394±0.093), (0.5525±0.0660) respectively. The differences between the expression of miR-181a and ATM protein in these two tissues were statistically significant (P<0.01). There was a negative corre-lation between miR-181a and ATM protein levels (r=-0.539, P< 0.01). Conclusion The expression of miR-181a is significantly up-regulated in gastric carcinoma tissues, whereas the ATM protein is markedly decreased.miR-181a may inhibit ATM expression by post-transcriptional gene silencing to restrain gastric carcinoma occurrence and progress.
展开▼
