武当山区市售重楼饮片中总皂苷含量分析研究

摘要

目的 比较武当山区市售重楼饮片中总皂苷的含量,考察医院、药店等医药市场上销售的重楼饮片质量情况,为中药重楼的生产、经销、临床使用和监管提供参考.方法 收集武当山区(以湖北省十堰市为主)医院、药店中重楼饮片样品,用高氯酸显色,在406 nm波长处使用紫外-可见分光光度法测定重楼饮片中总皂苷含量.结果 市售重楼饮片中重楼总皂苷的含量差异明显,收集到的23个样本中总皂苷含量最低的只有1.79％,最高达11.79％.结论 市售重楼饮片的质量参差不齐,会影响临床用药的安全性、有效性.需要加强对中药重楼的生产、经销监管.推动中药材规范化种植,以保证中药质量的稳定可控.%Objective To compare the content of total saponins in commercially available Paris polyphylla from Wudang Mountain Area to investigate the quality of herbal pieces purchased from hospitals,pharmacies and other pharmaceutical markets.It provides a reference for production,distribution,clinical application and regulation of Paris polyphylla.Methods Herbal pieces of the Paris polyphylla were collected from hospitals and pharmacies in Wudang Mountain Area (mainly in Shiyan,Hubei Province).The content of total saponins in Paris polyphylla was determined by an ultraviolet-visible spectrophotometer at 406 nm with perchloric acid as chromogenic reagent.Results The content of saponins in Paris polyphylla from 23 samples had obvious differences,the minimum was 1.79％,and the maximum was up to 11.79％.Conclusion The quality of Paris polyphylla is unstable which can affect the safety and efficacy of clinical application.The supervision of the production and marketing of Paris polyphylla need to be strengthened.Only by promoting the standardized planting of Chinese medicine materials,the stable quality of Paris polyphylla can be ensured.