不同采收期葛根中总黄酮含量的动态变化研究

摘要

目的 研究同一产地、不同采收期葛根中总黄酮含量的动态变化情况,确定葛根的最佳采收期.方法 采用紫外-可见分光光度法,取供试品用95％乙醇回流提取20 min,于250 nm处测定其吸光度,计算总黄酮含量.结果 不同月份采收的葛根中总黄酮的含量范围为3.79％～11.86％,以9～10月最高,4～5月最低.结论 以总黄酮为评价指标,葛根的最佳采收期应为9～10月,该结果可为葛根采收期的确定提供了可靠的科学依据.%Objective To study the dynamic change of total flavonoids content of Radix Puerariae in the same area and different harvest times,and determine the best harvest period of Radix Puerariae.Methods UV-Vis spectrophotometry was used,the test sample was extracted with 95％ ethanol for 20 min,the absorbance was measured at 250 nm,and the total flavonoid content was calculated.Results The content of total flavonoids in Radix Puerariae harvested in different months was 3.79％-11.86％,the highest from September to October,the lowest from April to May.Conclusion As to the total flavonoids as the evaluation index,the best harvest period of Radix Puerariae should be September to October,the results can provide a reliable scientific basis for determining root harvest period.