白介素-8、超敏C-反应蛋白及血管性假血友病因子联合检测在冠心病患者中的应用

摘要

目的:探讨白介素-8(IL-8)、超敏C-反应蛋白(hs-CRP)和血管性假血友病因子(vWF)在冠心病患者中的变化及临床意义.方法:选择医院诊断为冠心病的60例住院患者,根据美国心脏学学会及美国心脏协会(ACCF/AHA)诊断标准将其分为稳定型心绞痛(SAP)组(12例);不稳定型心绞痛(UAP)组(24例);急性心肌梗死(AMI)组(24);另选择30名健康查体者为健康对照组.各组均采集静脉血,采用化学发光法检测IL-8,采用散射比浊法检测hs-CRP;血浆采用免疫比浊法检测vWF.比较各组IL-8、hs-CRP和vWF的水平.结果:SAP组IL-8、hs-CRP和vWF水平检测结果分别为34.85 pg/ml、0.95 mg/dl和149.6％;UAP组IL-8、hs-CRP和vWF水平检测结果分别为68.45 pg/ml、2.3 mg/dl和187.45％;AMI组IL-8、hs-CRP和vWF水平检测结果分别为133 pg/ml、3 mg/dl和213.55％;SAP组、UAP组和AMI组分别与健康对照组结果6.5 pg/ml、0.015 mg/dl和52.8％相比,差异具有统计学意义(Z=-4.395,Z=-5.179,Z=-5.012;Z=-6.219,Z=-6.363,Z=-6.267;Z=-6.271,Z=-6.363,Z=-6.267;P<0.01);SAP组与UAP组比较,IL-8、hs-CRP差异有统计学意义(Z=-2.450,Z=-3.105;P<0.05);SAP组与AMI组比较,IL-8、hs-CRP和vWF差异有统计学意义(Z=-4.632,Z=-4.214,Z=-2.953;P<0.05);UAP组与AMI组比较,IL-8差异有统计学意义(Z=-3.64,P<0.01).结论:IL-8、hs-CRP和vWF三者均与冠心病的发生发展密切相关,并与病变的严重程度相关.%Objective:To investigate the changes and clinical significance of interleukin-8(IL-8), high-sensitivity C-reactive protein(hs-CRP) and von Willebrand factor(vWF) in patients with coronary heart disease.Methods: 60 patients with coronary heart disease were divided into three observation groups: stable angina pectoris(SAP) group(n=12), unstable angina pectoris(UAP) group(n=24) and acute myocardial infarction(AMI) group(n=24). Besides, 30 healthy persons were divided into the control group. After venous blood of every person were collected, chemiluminiscence method was applied to detect IL-8, the scattering turbidimetry was applied to detect hs-CRP, and immunoturbidimetry was applied to detect plasma vWF. The results of IL-8, hs-CRP and vWF among different groups were compared and analyzed.Results: The results of IL-8, hs-CRP and vWF in SAP group were 34.85pg/mL, 0.95mg/dL and 149.6％, respectively. And these indicators were 68.45pg/mL, 2.3mg/dL and 187.45％, respectively, in UAP group. And in AMI group, these indicators were 133pg/mL, 3mg/dL and 213.55％, respectively. While in control group, these indicators were 6.5pg/mL, 0.015mg/dL and 52.8％, respectively. The differences of IL-8, hs-CRP and vWF between each observation group and control group were statistically significant(Z=-4.395,Z=-5.179,Z=-5.012;Z=-6.219,Z=-6.363,Z=-6.267;Z=-6.271,Z=-6.363, Z=-6.267;P<0.01), respectively. And the differences of IL-8 and hs-CRP between SAP group and UAP group were statistically significant(Z=-2.450,Z=-3.105;P<0.05), respectively. The differences of IL-8, hs-CRP and vWF between SAP group and AMI group were statistically significant(Z=-4.632, Z=-4.214,Z=-2.953;P<0.05). And the difference of IL-8 between UAP group and AMI group was statistically significant(Z=-3.64,P<0.01).Conclusion: IL-8, hs-CRP and vWF are closely related to the happen and development of coronary heart disease, respectively, and all of them are related to the severity of the lesion.