UPLC同时测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量

摘要

建立UPLC同时测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚含量的方法,采用ACQUITY UPLCBEH-C 18色谱柱(2.1 mm×50 mm× 1.7 μm),乙腈和0.1％磷酸水溶液为流动相,采用梯度洗脱,流速为0.6 mL/min,检测波长为284nm.结果表明橙黄决明素、大黄素、大黄酚和大黄素甲醚分别在3.002～30.02 μg/mL、2.500～25.00 μg/mL、10.430～104.30 μg/mL和5.916～59.16 μg/mL的浓度范围内均有良好的线性关系(r=0.9999),回收率均大于95％,RSD均小于2％.该方法快速,准确,重现性好,可用于测定决明子中橙黄决明素、大黄素、大黄酚和大黄素甲醚的含量.%A method was established for simultaneously determining aurantio-obtusifolin, emodin, chrysophanol and physcione in Semen Cassiae by UPLC. The optimal conditions of separation and detection were achieved on an ACQUITY UPLC BEH-C18 column (2.1 mm×50 mm×l.7 μm). Mobile phase was acetonitrile and 0.1% phosphoric acid with gradient elution. Flow rate was 0.6 mL/min and detection wavelength was 284 nm. The results showed that there were good linear relationships (r=0.999 9) when the concentration of aurantio-obtusifolin, emodin, chrysophanol and physcione were in the range of (3.002-30.02)μg/mL, (2.500-25.00)μg/mL, (10.430-104.30)μg/mL, and(5.916-59.16)μg/mL respectively, the recovery rates were more than 95%, and the RSD were less than 2%. This method was simple, rapid, accurate, and reproducible, and it can be used for determining the concentration of aurantio-obtusifolin, emodin, chrysophanol and physcione in Semen Cassiae.