目的：探讨缺氧模型用于评定扩管药耳蜗效应的可行性，并确立适宜的低氧浓度及评定参数，以期为临床用药提供筛选方法。方法：分别在豚鼠7％，5％低氧供模型上，以ABR为指标，观察具有耳蜗扩管作用的丹参、川芎嗪和罂粟碱的耳蜗扩管效应是否得以体现。结果：与对照组相比，7％低氧供30min后，3种扩管药的耳蜗扩管效应均通过ABR阈移的显著降低得以体现，波I潜伏期及振幅的变化在各组间无显著差异；5％低氧供30min后，仅丹参和罂粟碱的耳蜗扩管作用得以证实。结论：低氧下听觉电生理反应模型可用于评价扩管药在耳蜗的效应，其适宜低氧浓度为7％，ABR阈移可用作评定药效时的观察指标。%Objective: To explore the possibility to evaluate the cochlear effect of vasodilator by the hypoxia model. Methods:theeffects of salvia mitiorribizae,ligustrazine and papaverine which had been testified to be improve the cochlear microcirculation on theABR were be o bserved in the guinea pig hypoxic modd(7％O2 or 5％O2). Results: Conpared with the control group, after animalswere subjected to hypoxia of 7％ oxygen for 30 minutes,the cochlear effect of three vasodilators were be confirmed by the marked reduce of the ABR threshold shift,the latency and the amplitude of wave I didn't appear and differen~ among groups. after ventilatedwith hypoxic gas of 5％ oxygen for 30 minute,only the salvia mitiorribizae and the papaverine's cochlear effect were be verified. Conclusion: the model of cochlear electrophysiology during hypoxia could be utilized to evaluate the cochlear effect of vasodilator, the appropriate hypoxia gas is 7％ oxygen,the threshold shift of ABR could be used as indexes.
展开▼
