[Objective] To explore the influencing factor on p16 protein expression and p16 gene mutation in NPC, and to investigate the correlation between NPC and p16 gene. [Methods] The expression of p16 protein in NPC by immunochemical technique; and the possible delection of p16 gene in exon 2 in NPC by multiplex-PCR.[Results] The delection rates of p16 protein expression were 55.3%(26/47)in NPC, 8% (2/25) cases in chronic inflammation of nasopharyngeal epithelium (CINE) respectively (P＜0.001); The deletion rates of p16 gene exon 2 were34% in NPC, Which were not detected in all the tumor-adjacent tissues(P＜0.001); The positive expression rate of p16 protein in NPC was higher than deletion rates of p16 gene. [Conclusion] We found the development of NPC was closely associated with the loss of p16 gene; and p16 protein down-expression may play an important role in the process.%目的探讨p16蛋白表达与p16基因突变在鼻咽癌发生中的作用,以确定两者之间的联系及意义.方法运用免疫组化二步法检测鼻咽癌中p16蛋白表达状况;采用多重PCR法检测鼻咽癌中p16基因第二外显子可能的缺失突变.结果p16蛋白表达缺失率:鼻咽癌组中55.3%(26/47),慢性鼻咽炎8%(2/25),鼻咽癌组中p16蛋白表达缺失率高于慢性鼻咽炎组(P＜0.001);p16基因第二外显子缺失突变:鼻咽癌组中纯合缺失突变率34%,而相应的癌旁组织未检出(P＜0.001);鼻咽癌p16蛋白表达缺失率高于p16基因外显子2缺失率.结论鼻咽癌的发生不仅与p16基因表达缺失和突变密切相关,而且p16蛋白表达下调可能是鼻咽癌发生发展的主要原因.
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