皮肤鳞状细胞癌组织中Axin1和Cyclin D1 mRNA的表达

摘要

目的：：检测皮肤鳞癌组织中Axin1和Cyclin D1 mRNA的表达,探讨其与皮肤鳞癌发生发展的关系。方法：采用实时定量PCR方法检测40例皮肤鳞癌和癌旁组织及40例正常人皮肤中Axin1和Cyclin D1 mRNA 的表达水平。结果：皮肤鳞癌组织中Axin1 mRNA表达<癌旁组织<正常皮肤组织,差异具有统计学意义(均P<0.05)。皮肤鳞癌组织中Cyclin D1 mRNA表达>癌旁组织>正常组织,差异具有统计学意义(均P<0.05)。皮损病理分化越差,Cyclin D1 mRNA表达量越高,Axin1 mRNA水平在皮肤鳞癌的病理分级中表达差异无统计学意义(均P>0.05)。结论： Cyclin D1和Axin1可能在皮肤鳞癌的发生发展中起一定的作用,尤其是Cyclin D1与肿瘤分化程度相关。%Objective: To detect the expressions of Axin1 and Cyclin D1 mRNA in cutaneous squamous cell carcinoma ( CSCC) in order to determine the relationship with the development of CSCC. Methods: The levels of Axin1 and Cyclin D1 mRNA in 40 samples of CSCC, adjacent skin of the CSCC lesions and 40 nor-mal control skin samples were detected by real-time quantitative polymerase chain reaction ( PCR) . Results:The level of Axin1 mRNA in lesions, adjacent skin and normal skin was reduced in a declined order, with sig-nificant differences (P<0.05) and the level of Cyclin D1 mRNA was increased in turn (P<0.05). The less differentiated the CSCC was, the higher level of Cyclin D1 mRNA among different pathological grades ( P<0.05) , but there was no significant difference in the level of Axin1 mRNA among different pathological grades ( P>0.05) . Conclusion: Axin1 and Cyclin D1 in CSCC may play an important role in the development of CSCC, especially Cyclin D1.