白介素－8与表皮生长因子对黑素瘤A375细胞增殖的影响

摘要

Objective:To determine the effects of IL-8 and epidermal growth factor ( EGF) on the prolif-eration of melanoma cell. Methods: The human melanoma cells A375 were divided into 9 groups: control group, EGF group, EGF combined AG1478 group, AG1478 group, IL-8 group, IL-8I group, IL-8 com-bined IL-8I group, EGF combined IL-8 group and EGF combined IL-8, AG1478 and IL-8I group. The pro-liferation of melanoma cell was detected by MTT. The level of EGFR, protein kinase B ( Akt) , mitogen activa-ted protein kinase (MAPK), phosphorylated protein kinase B (P-Akt), phosphorylated mitogen activated protein kinase ( p-MAPK) and phosphorylated epidermal growth factor receptor ( p-EGFR) were detected by western blot. The expression of EGFR and IL-8R mRNA levels were detected by qT-PCR. Results:The cell proliferation, the levels of proteins above and EGFR, and IL-8R mRNA levels in EGF group were higher than that in control group, EGF combined AG1478 group and AG1478 group ( P<0.05) . Those in IL-8 group were higher than that in control group, IL-8I group, IL-8 combined IL-8I group. Those in EGF combined IL-8 group was higher than that in IL-8 group and EGF group (P<0.05). Conclusion: IL-8 and EGF can pro-mote the proliferation of melanoma cells. There may be a synergistic effect between IL-8 and EGF, which may be related to the Akt and MAPK pathway.%目的：：明确IL－8与表皮生长因子( EGF)对黑素瘤细胞增殖的影响。方法：将黑素瘤细胞A375分为9组：EGF组；EGF＋AG1478组；AG1478组；IL－8组；IL－8I组；IL－8＋IL－8I组；EGF＋ IL－8组；EGF＋IL－8＋AG1478＋IL－8I组及对照组。采用MTT法检测各组细胞增殖情况；免疫印迹法检测EG-FR、蛋白激酶B、丝裂原活化蛋白激酶、磷酸化蛋白激酶B、磷酸化丝裂原活化蛋白激酶、磷酸化表皮生长因子受体的表达；实时荧光定量聚合酶链式反应检测EGFR与IL－8R mRNA水平的表达。结果：EGF组黑素瘤细胞增殖、上述蛋白的表达和mRNA水平显著高于对照组、EGF＋AG1478组和AG1478组。IL－8组显著高于对照组、IL－8I组和IL－8＋IL－8I组(均P<0.05)，EGF＋AG1478组显著高于EGF组和IL－8组( P<0.05)。结论： IL－8与EGF均能促进人黑素瘤细胞的增殖，它们之间可能具有相互协同作用，这种协同作用可能与AKT和MAPK等共同通路作用有关。