黄芩素对氧化应激状态下白癜风黑素细胞线粒体超微结构和功能的影响

摘要

目的:明确黄芩素对氧化应激状态下白癜风黑素细胞(PIG3V)线粒体超微结构和功能的影响.方法: 常规培养白癜风黑素细胞系PIG3V细胞,分为空白对照组、黄芩素组、H2O2组及黄芩素+H2O2组.电镜观察线粒体超微结构;MTT法检测线粒体活性;流式细胞术检测线粒体膜电位;荧光素酶报告系统检测细胞ATP含量;RT-PCR检测线粒体DNA拷贝数.结果: 与H2O2组比较,黄芩素+H2O2组线粒体结构损害轻,线粒体活性率、膜电位、ATP含量及DNA拷贝数高.结论: 黄芩素减轻氧化应激状态下白癜风黑素细胞线粒体结构和功能损害,增强细胞抗氧化能力.%Objective: To determine the effects of baicalein (BE) on the mitochondrial ultrastructure and function in stressed vitiligo melanocytes.Methods: The vitiligo melanocyte line (PIG3V) cells were cultured in vitro, and then, were divided into the blank control group, baicalein group, H2O2 group and baicalein+H2O2 group.The ultrastructure changes of mitochondria were observed under electron microscope.The mitochondrial activity, mitochondrial membrane potential (MMP), level of intracellular ATP, copy number of mitochondrial DNA (mtDNA) were detected by MTT, flow cytometry, ENLITEN ATP assay kit and real-time PCR (RT-PCR) respectively.Results: Compared with the H2O2 group, the level of the damage of mitochondrial ultrastructure was lower and the level of mitochondrial activity, MMP, intracellular ATP, copy number of mitochondrial DNA (mtDNA) were higher in the baicalein+H2O2 group.Conclusion: BE protects the structure and function of mitochondria from the damage of oxidative stress and enhances the anti-oxidative ability of PIG3V cells.