癌旁脂肪组织来源脂肪间充质干细胞的特征分析

摘要

Objective To investigate the characteristic by which human adipose-derived mesenchymal stem cel s(hAMSCs)derived from fat tissue adjacent to carcinoma. Methods Flow cytometry analysis for MSC-specific phenotype of AMSCs in the two sources of tissue. The transcripts of stemness-associated genes in hAMSCs were determined by a qRT-PCR assay. Results AMSCs were able to induce AMSCs to produce malignance-related genes,such as Oct4,Nanog,Rex1,IGF,HGF and SDF-1. Conclusion These findings thus suggest that the crosstalk between the microenvironment of cancer cel s and AMSCs has an implication in colon cancer malignancy.%目的：比较临床样本中肿瘤周围与肿瘤远端的脂肪组织来源的间充质干细胞生物学特征差异。方法流式细胞术鉴定两种来源脂肪间充质干细胞表面标志；Realtime-PCR检测两种来源脂肪间充质干细胞相关基因的表达变化。结果癌周脂肪组织中AMSCs表达更高的干细胞相关基因Oct4，Nanog，Rex1；生长因子IGF，HGF；趋化因子SDF-1。结论肿瘤微环境影响下的脂肪间充质干细胞可能增强结肠肿瘤细胞的侵袭能力。