梓醇对MPP+诱导的中脑多巴胺能神经元损伤的保护作用

摘要

为探讨梓醇对多巴胺能神经元的保护作用，本研究采用梓醇预处理中脑原代细胞，再给予MPP+（10uM）诱导多巴胺能神经元损伤。通过酪氨酸羟化酶（TH）的免疫细胞化学染色、脂质过氧化产物和细胞内活性氧类物质的检测，观察了梓醇对MPP+诱导的多巴胺神经元的影响。结果显示：经梓醇预处理可以明显对多巴胺能神经元起到保护作用，并且抑制脂质过氧化产物和活性氧自由基的生成。本研究结果提示，梓醇很可能成为氧化应激导致的神经细胞损伤性疾病的代替性化学药品。%The present study was to investigate the protective effects of catalpol on dopaminergic neurons were exposured to 1-methyl-4-phenylpyridinium (MPP+10uM)after pretreated with catalpol. The effests of catalpol on MPP+-induced oxidative stress in cultured dopaminergic neurons were observed by immunocytochemical staining for tyrosine hydroxylase(TH) and measuring intracellular reactive oxygen species(ROS). The results showed that catalpol increased neurons cellular viability and markedly attenuated MPP+-induced dopaminergic neurons death and reduced the content of ROS. These findings suggest that catalpol may be a candidate chemical for the treatment of oxidative stress-induced neurodegenerative disease.