正交试验法优化苹果皮中原花青素的提取工艺研究

摘要

The extraction technology of procyanidins from the apple pericarp was optimized. Based on single factor test, the effect of ethanol concentration, extraction time, extraction temperature, solid/liquid ratio ( g/mL) on the extraction rate of procyanidins was studied according to L,( 34 ) orthogonal test. The results showed that the order of the factors influencing the extraction by the water bath method were; ethanol concentration > solid/liquid ratio (g/mL) > extraction time > extraction temperature. The optimum extracting conditions of procyanidins from the apple pericarp by orthogonal test were: adding 10% ( v/v) of 60% alcohol and extracting for 120 minutes at 65℃. The extraction of procyanidins was 2. 82mg/g under the above conditions.%对苹果皮中原花青素的提取工艺条件进行了优化,在单因素的基础上,采用L9( 34)正交试验设计,研究乙醇浓度、料液比、提取时间和提取温度对苹果皮中原花青素得率的影响.结果表明,对苹果皮原花青素得率的影响顺序是:乙醇浓度＞料液比＞提取时间＞提取温度,有机溶剂提取苹果皮原花青素的最佳工艺条件是:乙醇浓度60％,料液比1∶10 (g/mL),提取温度65℃,提取时间120min,原花青素得率达到2.82mg/g于皮.