试验采用了有机溶剂浸提和氢氧化钾甲醇溶液皂化的方法,研究了万寿菊叶黄素的提取与皂化的工艺条件.试验考察了提取剂种类、提取温度、提取时间、料液比对提取效果的影响,以及皂化液种类、皂化液浓度、皂化液用量、皂化温度、皂化时间、对皂化的影响.通过单因素试验确定的提取和皂化工艺条件为:提取剂为无水乙醇:石油醚(沸程为60℃～90℃)(2:1),料液比为1:100,提取温度45℃,提取时间80min,皂化液浓度20％,皂化液用量0.2mL,皂化温度40℃,皂化时间80min.选用乙醇—石油醚混合溶剂作为提取剂,具有价格便宜、低毒、沸点低、溶剂回收成本较低、生产安全性高等优点.该提取剂用于工业生产中提取叶黄素是可行的.%With the method ot organic solvent extraction and mehtanol solution ot potassium hydroxide saponuication, this research studied the extraction and saponification process conditions of lutein in marigold flower. Research examined the effects of extraction agents types, extraction temperature, extraction time, and ratio of material to liquid on extraction, as well as the effects of saponification solution types, saponification solution concentration, saponification liquid dosage, saponification temperature and saponification time on saponification. On the base of single - factor test, the optimized conditions of extraction and saponification were: extraction agents was ethanol: petroleum ether (boiling range of 60 °C~90°C ) (2:1), expected liquid ratio was 1 : 100, extraction temperature was 45°C, extraction time was 80min, saponification solution concentration was 20% , the amount of saponification solution was 0. 2mL, saponification temperature was 40 "C , saponification time was 80min. The use of ethanol - petroleum ether mixed solvent as extraction agent has the advantages of cheap, low toxicity, low boiling point, low solvent recovery costs, safe production. This extractant agent to extract lutein in industrial production was feasible.
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