荧光假单胞菌JD1202利用大米淀粉水解糖连续发酵生产2-酮基-D-葡萄糖酸

摘要

A continuous fermentation process of 2 -keto -D -gluconic acid (2KGA) production from rice starch hy-drolysate was developed using Pseudomonas fluorescens JD1202. The effects of starting point of continuous fermenta tion, dilution rate and feeding glucose concentration on 2KGA production were investigated. Results showed that the dilution rate and feeding glucose concentration had a significant effect on 2KGA production performance. When the dilution and media composition were at a stable level, the fermentation can start at any point, there had no significance of the concentrations of cell, substrate and product. The optimal operating factors were obtained as: 0.065 h -1 of dilution rate and 170. 0 g/L of feeding glucose concentration. Under these conditions, the steady state produced 2KGA at the concentration of 130. 34 g/L, average volumetric productivity of 8. 47 g/L h and the fermentation conversion rate of 89. 46%. In conclusion, this efficient continuous fermentation process by the strain Pseudomonas fluorescens JD1202 is competitive for industrial production 2KGA from rice starch hydrolysate.%研发荧光假单胞菌JD1202转化大米淀粉水解糖为2-酮基-D-葡萄糖酸的连续发酵工艺.考察了连续发酵起点、稀释率和补料培养基中葡萄糖浓度对2-酮基-D-葡萄糖酸生产的影响.结果表明,稀释率和葡萄糖浓度对2-酮基-D-葡萄糖酸发酵具有显著影响；在稀释率和补料培养基成分保持不变的条件下,2-酮基-D-葡萄糖酸大量合成期的任何时段均可作为连续发酵的起点,其细胞浓度、残糖浓度和2-酮基-D-葡萄糖酸浓度均稳定在一定范围.较适的连续发酵操作条件为:稀释率0.065h-1,补料培养基中葡萄糖的质量浓度为170.0g/L.在此条件下,连续发酵达到稳态时发酵液中2-酮基-D-葡萄糖酸的质量浓度为130.34g/L,生产强度平均为8.47g/L·h,发酵转化率为89.46％.连续发酵工艺是一种高效的2-酮基-D-葡萄糖酸生产方法,有望应用于工业生产中.