高效液相色谱法测定食品中桔青霉素的不确定度分析

摘要

采用高效液相色谱法对食品中桔青霉素残留量测定的不确定度进行评估.依据JJF 1135-2005《化学分析测量不确定度评定》和JJF 1059.1-2012《测量不确定度评定与表示》规定的测量不确定度的基本方法,建立数学模型,分析食品中桔青霉素的不确定度来源,通过对不确定度分量进行量化和合成,得出当食品中桔青霉素残留量为154 μg/kg时,其扩展不确定度为8.6 μg/kg (k=2).评定结果表明,影响检测结果的主要因素为标准曲线拟合、样品前处理过程和测量重复性等.%Evaluation on uncertainty of determination of citrinin in foods by high performance liquid chromatography was practiced.Based on JJF1135-2005 Evaluation of Uncertainty in Chemical Analysis Measurement and JJF1059.1-2012 Evaluation and Expression of Uncertainty in Measurement,a mathematical model was established to evaluate the uncertainty of determining the citrinin residues in foods.The possible uncertainty sources were analyzed and each component of uncertainty was quantified and combined.The results showed that the expended uncertainty was 8.6 μg/kg with 154 μg/kg (k=2)residues,and the configuration of standard curve fitting,sample pretreatment and measurement repeatability were the main sources of uncertainty.