Process for quantitative analysis of nargenicin in Nocardia sp. CS682 cultures by high performance liquid chromatography

摘要

The present invention is quantified using high performance liquid chromatography put in the furnace to carry Jenny Cardia in microbial culture relates to a method of analysis , and more specifically to the method of the present invention includes the steps of extraction with ethyl acetate from the carry Jenny put microorganism culture broth , and the solvent a (0.5 M potassium phosphate pH 2.5- di- water : acetonitrile = 80: 20 ) and a solvent using a B (100% acetonitrile) comprises the step of step of detecting at 215 nm , and quantitative analysis by high performance liquid chromatography . The method of the present invention provides a method for rapid and precise analysis of the amount present in the free God carry Jenny cell culture medium , is considered to make usefully applied to other cell culture analysis of water containing similar compounds .