响应面优化盐析萃取万寿菊花中叶黄素的研究

摘要

采用丙酮/磷酸氢二钾两相盐析体系萃取万寿菊花中叶黄素,确定最优盐析体系组成为27％(w/w)丙酮/18％(w/w)磷酸氢二钾.通过单因素实验探讨了提取温度、静置时间、料液比等对叶黄素在两相间分配行为的影响,并运用响应曲面法研究磷酸氢二钾质量分数、料液比、温度三个因素对万寿菊花中叶黄素得率的影响,得出三个考察因素最优工艺参数,即,磷酸氢二钾质量分数为20％,料液比1∶40,萃取温度42℃.在此条件下,叶黄素富集于上相丙酮相,得率为6.23 mg/g.室温下采用2,2-二苯基-1-苦基肼(DPPH)法测定两相盐析萃取物的IC50为0.34 mg/mL,比索氏及超声辅助提取物具有更强的抗氧化活性.%Salting-out extraction system composed of acetone/K2HPO4 was used to separate lutein from marigold flower.Salting out to determine the optimal system consists of 27％ acetone (w/w) /18％ (w/w) K2HPO4.The effects of three factors on the extraction rate of lutein in marigold flower were studied by response surface methodology.The optimal parameters of three factors were obtained,namely,the quality of dipotassium hydrogen phosphate concentration was 20％,the ratio of material to liquid was 1 ∶ 40,and the extraction temperature was 42 ℃.Under these conditions,the lutein was enriched in the phase of acetone phase,the yield was 6.23 mg/g.The antioxidant activity of marigold flower extracts were assayed with radical-scavenging ability (IC50) of 2,2-diphenyl-1-picrylhydrazyl at room temperature.The marigold flower extracts by salting-out extraction showed lower IC50 (0.34mg/mL) than that by soxhlet and ultrasound-assisted extraction.