角蛋白酶制备羽毛蛋白粉的工艺研究

摘要

为研究角蛋白酶对羽毛蛋白粉的降解效果，采用不同的酶解温度、初始酶解液pH、酶添加量和酶解时间对羽毛蛋白粉进行酶解，并利用正交试验对酶解条件进行优化。结果表明：每克羽毛粉加入角蛋白酶0.03 g，酶解液初始pH为7，在35℃下酶解36 h。羽毛分解率达到50.5%，酶解液可溶性蛋白含量为225.7 mg/L，而酶解羽毛蛋白粉的胃蛋白酶体外消化率为23%，显示了角蛋白酶具有很好的酶解效果。%To research the degradation effects of keratinase on feather protein powder,different reaction tempera-ture,initial hydrolyzate pH,enzyme dosage and hydrolysis time were studied by orthogonal test.The results suggested that the optimal condition was keratinase adding level of 0.03 g,hydrolyzate initial pH of 7 and at 35 ℃under enzymatic 36 h. Feather decomposition rate was 50.5% and the soluble protein hydrolyzate content was 225.7 mg/L,and enzymatic di-gestibility of the feather protein powder in vitro was 23%.It was concluded that the enzymatic hydrolysis effects of the keratinase on feather protein powder was good.