分子荧光法快速测定饲料中的金霉素

摘要

In order to develop the rapid determination of chlortetracycline content in feed,the samples were deter-mined by molecular fluorescence spectrometry after extraction with ultrasonic technology.The results showed that the opti-mal excitation wavelength was 350 nm for chlortetracycline hydrochloride,emission wavelength was 421 nm,the conversion time was 60 min,transformation temperature was 40 ℃,pH was 11.50,ultrasonic temperature was 60 ℃,ultrasonic power was 160 W and the material liquid ratio was 1∶50.The regression equation of work curve was Y=798.32+21.208X,the lin-ear correlation coefficient (r) equalled 0.9996,detection limit was 0.544 ng/mL,quantitative limit was 1.813 ng/mL.The standard addition recovery rate was between 92.00% and 103.83%,and relative standard deviation (RSD) was less than 1%(n=11).%为快速测定饲料中金霉素含量，将实验样品采用超声波浸提后，用分子荧光法快速测定。结果表明，盐酸金霉素的最佳分析条件为：激发波长350 nm，发射波长421 nm，转化时间60 min，转化温度40℃，pH 11.50，超声温度60℃，超声功率160 W，料液比1∶50。工作曲线的回归方程为Y=798.32X+21.208，相关系数r=0.9996，检出限为0.544 ng/mL，定量限1.813 ng/mL；样品加标回收率为92.00%～103.83%，相对标准偏差（RSD）小于1%（n=11）。