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METHOD OF QUANTITATIVE DETERMINATION OF FLAVONOIDS IN VEGETABLE RAW MATERIAL BY FLUORIMETRIC METHOD

机译:荧光法定量测定蔬菜原料中黄酮的方法。

摘要

FIELD: medicine, pharmaceutics.;SUBSTANCE: invention describes method of quantitative determination of flavonoids in vegetable raw material, includes exhaustive extraction of vegetable raw material with ethyl alcohol, concentration, optimal for each type of raw material, obtaining alcohol extract, containing flavonoids, addition of aluminium chloride solution to extract, where fluorimetry of obtained solution is performed at excitation wavelengths and registration wavelengths which are individual for each particular compound of flavonoid nature, relative to alcohol aluminium chloride solution in comparison with standard solutions of individual flavonoids, processed in the same conditions as extraction from raw material.;EFFECT: invention ensures quantitative determination of individual flavonoids in vegetable raw material.;3 ex
机译:领域:本发明描述了定量测定蔬菜原料中黄酮类化合物的方法,包括用乙醇彻底提取蔬菜原料中的黄酮类化合物,浓缩,针对每种原料的最佳浓度,获得含黄酮类化合物的醇提取物,添加氯化铝溶液进行萃取,其中所获得溶液的荧光测定是在激发波长和配准波长下进行的,这些波长和黄酮类化合物相对于醇氯化铝溶液是与每种类黄酮标准溶液相比在每种波长下对于类黄酮性质的特定化合物而言都是独立的效果:本发明确保了对蔬菜原料中单个类黄酮的定量测定。3ex

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