动物双歧杆菌A6小试高密度发酵培养基的优化研究

摘要

The strain for this study isBiifdobacteriumanimalissubsp.LactisA6, with MRS culture medium based, using orthogonal test method, viable count as the evaluation index, to optimize adding quantity of cultivate medium carbon source, nitrogen source and inorganic salt.Final determine the viable count of the best culture medium formula: Peptone 10.0g/L, Yeast Extract Powder 7.5g/L, beef extract 5.0g/L, tomato juice 20g/L,D-Glucose 20.0g/L, Sodium acetate anhydrous 7.5 g/L,Magnesium sulfate heptahydrate 0.9g/L, Manganese sulfate monohydrate 0.2g/L, di-Ammonium hydrogen citrate 2.0g/L, di-Potassium hydrogen phosphate trihydrate 2.0g/L,Tween 80 1.0 g/L, L-Cysteine hydrochloride 0.5g/L.Through 50L fermenter small-scale test to examine the effect of optimized culture. The results showed thatBifidobacterium animalissubsp.Lactis A6 about at 12h reached stationary phase, cell concentration reached 6.0×109 cfu/mL, the optimized culture effect is better, has the value of practical application.%动物双歧杆菌A6是一株具有优良功能的益生菌。为了实现菌株的产业化应用，本研究对该菌株高密度发酵培养基进行了优化。以动物双歧杆菌A6的活菌浓度为评价指标，以MRS培养基为基础培养基，利用正交试验对培养基中氮源、促生长因子以及无机盐添加量进行了优化，确定活菌浓度最佳的培养基配方为：蛋白胨10.0g/L，酵母粉7.5g/L，牛肉膏5.0g/L，西红柿汁20g/L，葡萄糖20.0g/L，无水乙酸钠7.5g/L，七水合硫酸镁0.9g/L，一水合硫酸锰0.2g/L，柠檬酸氢二铵2.0g/L，磷酸二氢钾2.0g/L，吐温-801.0g/L，L-半胱氨酸盐酸盐0.5g/L。对优化完成的培养基进行50L发酵罐小试试验，结果表明动物双歧杆菌A6约在12h达到稳定期，菌体浓度可以达到6.0×109cfu/mL，与基础培养基培养结果（1.6×109cfu/mL）相比有显著的提高，具有实际应用价值。