本研究建立了可替代现有黄曲霉毒素M1免疫亲和柱的液液萃取方法，优选了合适萃取液以及萃取条件，使操作方法简单易行。具体步骤为：选择优化高效液相色谱仪测定条件，包括流动相类型、流速等条件，然后用优化的检测方法对加标后的黄曲霉毒素M1及其加标回收率、相对标准偏差、相关系数进行测定。结果显示，待测组分线性关系良好，加标回收率分别在91.2%~105.5%，相对标准偏差在1.90%~2.84%之间，纯牛奶和乳粉的线性相关系数分别为0.9979、0.9951。表明本方法结果准确、重复性好，可用于牛乳制品中黄曲霉毒素M1含量的测定。%A method was developed for replacing the existing Liquid-liquid extraction of aflatoxin M1 immune affinity column, high performance liquid chromatography test condition was optimized, including the type of mobile phase and lfow rate. Then the recovery rates,relative standard deviation and the correlation coeffcient after standard addition were determined. Under the optimum conditions, good linear relationships were obtained for AFM1, the spiked recoveries were in the range of 91.2%~105.5% with relative standard deviations between 1.90%-2.84%.The correlation coeffcients were 0.9979 (milk) and 0.9951(milk powder). This method was simple, accurate, reproducible and could be used for determination content of alfatoxin M1 in milk products.
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