牛支原体快速PCR检测方法的建立r及其应用

摘要

The aim of this study was to develop a rapid and direct PCR method to detect Mycoplasma bovis from diseased materials. A pair of primers were designed according to the sequence of 16S rRNA gene of Mycoplasma bovis, and the system of direct PCR amplification was prepared. After simple treatment, the samples were added into the system for amplification and electrophoresis. A 1390 bp fragment was amplified, and the specificity and sensitivity were tested. The results showed that the method was specific and sensitive. The method was applied to detect clinical samples, and the detection rate was 100% conform with the traditional PCR. The method can directly detect Mycoplasma bovis from clinical samples and can be used for the detection of a large number of clinical samples of Mycoplasma bovis.%本试验旨在建立一种可直接从病料中快速检测牛支原体的PCR方法,并且应用于临床.根据牛支原体16S rRNA基因设计一对引物,配制PCR扩增体系,样品经简单处理后加入体系进行扩增,最终扩增出1390bp片段,进一步的试验证明该方法具有良好的特异性和敏感性.临床样品检测结果显示,该方法的检出率与传统PCR的符合率为100%.试验表明,该方法能快速直接从临床样品中检测出牛支原体,可用于牛支原体的大量临床样品的检测.