为达到选育甜酸味品系、缩短育种周期、提高选择准确率的目的，本研究基于简化基因组测序（SLAF-seq）的选择性基因型鉴定和集群分离分析（Super-BAS），结合生物信息学方法，以‘风味4号’甜瓜F2群体甜味、酸味、不甜不酸3个极端表型群体为试验材料，精细定位甜瓜含糖量、酸度性状相关基因并开发功能性标记。结果显示：（1）将甜瓜含糖量、酸度性状定位在双亲基因组；（2）获得1个含糖量性状相关候选基因，3个酸度性状相关候选基因；（3）针对候选基因开发出1个含糖量性状相关基因功能性分子标记SLAF18745-S01和3个酸度性状相关基因功能性分子标记SLAF36334-S02、SLAF50072-S03和SLAF31212-S04。本研究开发的功能性分子标记为甜瓜品质性状基因的分子标记辅助选择提供了重要的标记基础。%SLAF-seq-based selective genotyping and bulked segregant analysis(Super-BSA),together with bioinformatic methods were performed in three extreme phenotypes populations(sweetness,acidity and neither sweet nor sour)from the F2 population of a flavorful melon(Cucumis melo L.)‘Fengwei No. 4’;fine mapping of its genes related to sugar content and acidity traits was achieved and functional markers were developed. Results showed that:(i)both sugar content and acidity traits were mapped in parental genome;(ii)one sugar content trait-related candidate gene and three acidity trait-related genes were obtained;(iii)one sugar content(SLAF18745-S01)and three acidity(SLAF36334-S02,SLAF50072-S03 and SLAF31212-S04)trait-related gene functional molecular markers were developed based on the candidate genes. In conclu⁃sion,the functional molecular markers developed in this study provide an important marker foundation for molecular mark⁃er-assisted selection of quantitative trait loci of melons,which can reduce breeding cycles and improve the accuracy of selec⁃tion.
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