血清AFP、AFP-L3及GP73三者联合检测在原发性肝癌诊断中的应用探究

摘要

Objective To investigate and analyze the application value of combined detection of serum alpha (AFP), alpha fetal protein (AFP-L3) and Golgi protein (GP73) in the diagnosis of primary hepatic cancer.Methods We Selected 40 cases of HCC patients (group A), 40 cases of benign liver disease patients(group B)and 40 cases of healthy volunteers (group C) as the research object. The serum levels of AFP, AFP-L3 and GP73 were detected by electrochemical luminescence, micro column and enzyme linked immunosorbent assay (ELISA). Results AFP, AFP-L3, GP73 were not detected in the serum of patients with C group.But group A and B were signiifcantly higher than those in the control group, and the difference was statistically significant (P<0.05). The positive rates of AFP-L3, GP73 in group A were 70%and 77.5%. Compared with the positive rate of AFP serum detection, the difference was statistically signiifcant (P<0.05). But significantly lower than the positive rate of AFP,AFP-L3 and GP73,the difference was statistically significant (P<0.05). The sensitivity and specificity of the combined detection of AFP,AFP-L3 and GP73 were 96.4%,88.4%,they were better than those of the above indexes,the difference was statistically signiifcant (P<0.05). Conclusion The combined detection of AFP,AFP-L3 and GP73 had important significance in the diagnosis of primary hepatic cancer, which could signiifcantly improve the patient positive test results, while ensure a better sensitivity and speciifcity.%目的：探讨和分析血清甲胎蛋白（AFP）、甲胎蛋白异质体（AFP-L3）、高尔基体蛋白（GP73）三者联合检测在原发性肝癌诊断中的应用价值。方法选取40例肝癌患者（A组）、40例肝脏良性疾病患者（B组）和40例健康志愿者（C组）作为此次研究的对象，分别采用电化学发光法、微量离心柱法和酶联免疫吸附法（ELISA）对上述对象血清中AFP、AFP-L3及GP73进行检测。结果 C组血清均未检出AFP、AFP-L3、GP73，A组和B组上述指标血清含量均明显高于对照组（均P＜0.05）；A组AFP-L3和GP73血清检测阳性率分别为70%和77.5%，与AFP血清检测阳性率比较差异具有统计学意义（P＜0.05），但显著低于AFP、AFP-L3和GP73联合检测的血清阳性率（P＜0.05）；AFP、AFP-L3及GP73三者联合检测的敏感度、特异度分别为96.4%、88.4%，显著优于上述指标单独检测的敏感度、特异度（均P＜0.05）。结论 AFP、AFP-L3及GP73三者联合检测在原发性肝细胞癌联诊断中具有重要意义，提高阳性检出率的同时还具有较好的敏感度和特异度。