2株酵母菌单倍体原生质体形成与再生条件的研究

摘要

The conditions of formation and regeneration of protoplast from two high-alcohol-yield Saccharotnyces cerevisiae haploid strains GGFS16 and GJ2008 were optimized. The optimum conditions were as follows: the haploid cultured for 8h, hydrolyzed with 1.5% snailase at 37^ for 30 min after pretreatment by 0.2% mercaptoethanol and 0.1% EDTA-Na2; 170g/L sucrose were used as osmotic stabilizer. Under these conditions, the formation rate of protoplast from the GGFS16 and GJ2008 haploid strains reached 98.14% and 97.19%, respectively. The regeneration rate reached 17.29% and 15.78%, respectively..%对酒精高产酿酒酵母GGFS16和GJ2008单倍体原生质体的形成与再生条件进行了研究.结果表明,在菌龄8h、o.2％p-巯基乙醇和0.1％ EDTA-Na2预处理20min、1.5％的蜗牛酶37℃处理30min以及使用浓度170g/L的蔗糖作为渗透压稳定剂的条件下,GGFS16和GJ2008单倍体原生质体的形成率和再生率均达到最大.形成率分别是98.14％和97.19％,再生率分别是17.29％和15.78％.