枯草芽孢杆菌C3产抗菌物质发酵培养基的优化

摘要

该文利用对黑曲霉孢子萌发有抑制作用的枯草芽孢杆菌C3,研究优化产抗菌物质的发酵培养基组分.在碳源、氮源、C/N单因素多水平试验结果的基础上,设计Na+、Mg2+、K+度3因素3水平L9(33)正交试验,研究无机盐离子对枯草芽孢杆菌产抗菌物质的影响.结果表明,碳源为1％葡萄糖,氮源为蛋白胨和酵母浸粉各1.5％,碳氮比为1∶3,NaCl为0.5％,KH2PO4为0.1％,MgSO4·7H2O为0.15％时,枯草芽孢杆菌产抗菌物质的抑菌效果明显,对霉菌孢子萌发的抑制率提高了118.64％.%Using Aspergillus niger spore germination of an inhibition of Bacillus subtilis C3, this paper optimizes antifungal substances of fermentation components for research. Based on the test results of carbon source, nitrogen source, C/N single factor with many levels, we design three factors three levels L9(33) orthogonal test of Na+, Mg2+, K+ concentration to test the influence of inorganic salt ion to Bacillus subtilis antibacterial material. The results show that when the carbon source is 1% glucose, nitrogen source is peptone and yeast extract 1.5% each, C/N is 1:3, NaCl is 0.5%, KH2PO4 is 0.1%, MgSO4?H2O is 0.15%, the antibacterial effect from Bacillus subtilis antibacterial substance is obvious and the inhibition rate of fungus spore germination increases 118.64%.