The duck infectious serositis is one of the most acute and septic infectious with a high mortality, which severely affect the development of ducks industry. All kinds of conditions were screened in study on infecting method of serositis in duck. Ducks of six-week old were injected with Pasteurella anatipestifer by abdominal cavit. On the basis of the experiment of bacteria dose of infection,the most suitable experimental infectious dose of bacteria was 0. 5 mL (4. 5×109CFU/mL). Injected ducks began to sicken after five hours.and the mortality came to 80% in 3 to 5 days. The clinical symptoms, the pathology examination and etiology identification are in line with the requirements of disease model replication. The method can provide theoretical basis for pathology study and screening for new drugs.%鸭传染性浆膜炎是鸭最常见的急性、败血性传染病之一,死亡率高,严重影响到养鸭业的发展.本试验通过对鸭传染性浆膜炎疾病模型的建立进行条件筛选,确立对6周龄雏鸭采用腹腔注射0.5 mL4.5×109CFU/mL细菌浓度的鸭疫里默氏杆菌,5 h后鸭开始发病,3～5 d死亡率达80％,临床症状、病理学检查及病原学鉴定均符合该疾病模型复制的要求.本试验结果可以为该病的病理学研究及新药筛选提供理论依据.
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