犬瘟热病毒贵州分离株N基因的克隆及序列分析

摘要

根据GenBank中发表的犬瘟热病毒Onderstepoort株N蛋白基因序列设计两对特异性引物,采用RT-PCR扩增犬瘟热病毒贵州分离株( CDV- GZ1)的N基因,并进行克隆与序列分析.结果显示,CDV-GZ1株N基因的ORF全长1572 bp,其编码氨基酸序列与国外Shuskiy株和01-2689株的同源性分别为98.9％和97.1％,与部分国内分离株同源性在95％以上,说明N蛋白是保守性较强的结构蛋白.%Two pairs of primers were designed and synthesized based on the sequence of the Onderstepoort strain of canine distemper virus reported in GenBank.and the N gene was amplified by reverse transcription polymerase chain reaction (RT-PCR) from CDV-GZ1 strain in Guizhou. The amplified fragment was cloned and analyzed. The results showed that the length of N gene was 1572 bp. The homology of coding amino acid between this strain and CDV Shuskiy and 01-2689 strain was 98. 9% and 97. 1% respectively,while above 95% among other domestic strain. It illustrated that N protein have highly conservation property.