副猪嗜血杆菌的分离鉴定及药敏试验

摘要

One strain of bacteria was isolated from Liaoning samples of pigs with suspected multiple subserosal inflammation and arthritis in 2011. We used the lungs, pericardial fluid, blood from diseased samples via bacterial isolation and culture techniques, biochemisty tests in addition to PCR identification finally indicated that the bacteria belonged to Hemophilus parasuis. Then drug sensitive test and analysis were performed on the strain named HPSLN111013. The results showed that the strain on some aspects such as morpha, cultural character and biochemisty tests were in accord with separate strains from other areas in China. Then drug sensitive test revealed that the isolate was the most sensitive to florfenicol, ceftiofur and lomefloxa-cin. These results supplied a foundation for the future research of Hemophilus parasuis 's serological and gene typing. Ascertaining the infection source and genetic variation of Hemophilus parasuis provided a basis of prevention and control measures of this disease in Liaoning province.%2011年从辽宁省采集以多发性纤维素性浆膜炎为主要特征的病猪肺脏、心包液、血液等病料,通过分离培养、生化试验和PCR检测等方法确认分离到1株副猪嗜血杆菌Hps LN111013,同时应用多种药物对该菌株进行了药物敏感性试验及分析；结果表明该菌株在形态、培养特性和生化试验等方面与国内其他地区所分离到的菌株基本一致,纸片法药敏试验证明本菌对氟苯尼考、头孢噻呋、洛美沙星最为敏感.本研究为进一步确认辽宁省副猪嗜血杆菌流行菌株的血清型和基因型,查明其传染来源和遗传变异情况奠定了基础,为制定辽宁省副猪嗜血杆菌病防控措施提供依据.