猪细小病毒的分离与鉴定

摘要

本试验从福建省某猪场疑似猪细小病毒病死胎的淋巴结、肝脏中分离到1株病毒.病料接种PK-15细胞36 h后出现了圆缩、集聚、脱落等细胞病变,猪细小病毒阳性血清能特异性地中和该分离病毒.根据已发表的细小病毒(PPV) VP2基因的序列设计并合成了一对引物,采用PCR方法可扩增531 bp DNA片段.测序结果表明,分离株VP2基因与NCBI公布的NADL-2株的同源性高达99.2％,证实分离的病毒株为猪细小病毒.为进一步开展该病毒致病机理、流行病学、诊断研究与疫苗免疫等奠定了基础.%One local strain of porcine parvovirus was isolated from the dead fetus in Fujian province. The isolated virus could cause typical cytopathogenic effect (CPE) in PK-15 cell at hour 36 after infection such symptoms as shrunk, aggregated, and detached. The isolated virus could be neutralized by the positive sera. The DNA extracted from the virus isolate was subjected to polymerase chain reaction (PCR) analysis, a set of primer which can be used to amplify 531 bp fragment in the gene of PPV was designed. The homology of VP2 gene sequence between the isolated viruses and NADL-2 from GenBank was 99. 2% , it was indicated that the isolated virus was porcine parvovirus (PPV). In conclusion, this study laid the foundction for further development of the pathogenic mechanism of virus, epidemiology, diagnostic studies and vaccine.