为确定王不留行对奶牛乳蛋白合成信号转导通路的影响,本试验通过水浸提方法制备王不留行提取液,采用Western blotting方法分别检测经王不留行水浸提液、雌激素及催乳素处理的泌乳期奶牛乳腺上皮细胞中乳蛋白合成信号分子的表达变化.结果显示,王不留行水浸提液、雌激素及催乳素都可以促进p-STAT5、pl00、GAS、S6K1及p mTOR的表达,进而促进乳蛋白合成,且添加蛋氨酸可以促进这一作用.提示,王不留行具有和雌激素、催乳素相似的作用,并在转录和翻译水平调节泌乳基因表达.%To study the effect of Vaccaria segetalis on cellular signal transduction pathway associated with milk protein synthesis in dairy cow, water extraction of Vaccaria segetalis was prepared. Western blotting was used to study the changes of the signal molecules associated with milk protein synthesis of dairy cow mammary gland epithelial cells (DCMECs), treated with estrogen (ER), prolactin (PRL) and Vaccaria segetalis. The results showed that the Vaccaria segetalis extraction liquid, ER and PRL could promote milk protein synthesis and up-regulate the expression of p-STAT5, p100, GAS, S6K1 and p-mTOR, furthmore, methionine could enhance this action. These datas suggested that Vaccaria segetalis had a similar role with ER/ PRL and up-regulated expression of the genes of DCMECs for lactation on the translational and transcriptional level.
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