鸽Ⅰ型副黏病毒与沙门氏菌、大肠杆菌混合感染的诊断

摘要

从珠三角某鸽场病死鸽中无菌操作采取心血、肝脏、脑、脾脏,接种于麦氏培养基和木糖—赖氨酸—脱氧胆酸琼脂培养基,经细菌纯化、镜检、生化试验、PCR检测和药敏试验,结果显示30只病鸽有8只分离到沙门氏菌,阳性率为26.7％；10只分离到大肠杆菌,阳性率为33.3％；其中6只同时分离到沙门氏菌和大肠杆菌,混合感染率为20.0％.经病毒分离培养、PCR检测,结果显示从6个混合样品中分离到5株副黏病毒,分离率为83.3％.结果表明,该鸽场鸽群发生了副黏病毒、沙门氏菌、大肠杆菌混合感染.本试验对珠三角鸽场近年较常发生的、以乳鸽较严重发病死亡的典型疫情作了较明确的诊断.%It was isolated from blood, liver, brain, spleen of mortality pigeons in a pigeon farm in the pearl river delta region, inoculated on MacConkey medium and xylose, lysine, deoxycholate medium, after bacterial purification, microscopic examination, biochemical test, PCR test and drug sensitivity test, results showed that, 8 strains of Salmonella were isolated from 30 samples, the isolation rate was 26. 7%, 10 strains of E. coli were isolated, the rate was 33. 3% , 6 pigeons were infected Salmonella and E. coli, mixed infection rate was 20. 0%. By virus isolation and PCR testing, results showed that 5 avian paramyxovirus of the 6 samples were isolated, the isolation rate of 83. 3%. The incidence of death was caused by mixed infection of pigeon paramyxovirus, Salmonella and E. coli