小花棘豆中毒对和田羊母羊繁殖器官的影响

摘要

试验旨在探讨小花棘豆(Oxytropis glabra DC)中毒对和田羊母羊繁殖器官指数、性激素水平和相关基因mRNA表达量的影响.以小花棘豆中毒和田羊母羊为研究对象,采集血清后屠宰,采集试验羊的丘脑、垂体和卵巢组织,测定其脏器系数,检测血清中促性腺激素释放激素(GnRH)、促卵泡素(FSH)、促黄体素(LH)、雌二醇(E2)和孕酮(P4)含量的变化,并检测各组织中相关繁殖基因的表达.结果显示,小花棘豆中毒和田羊母羊丘脑、垂体和卵巢指数均极显著升高(P＜0.01),且卵巢表面卵泡数显著下降(P＜0.05),HE染色表明丘脑神经元细胞固缩、浓染;垂体中细胞核变形、浓染,胞浆减少;卵巢中初级卵母细胞溶解、消失,间质血管扩张.小花棘豆中毒和田羊母羊血清中GnRH、FSH、LH、E2和P4含量均极显著下降(P＜0.01),丘脑中Kiss-1、GPR54、ERα mRNA,垂体中GnRHR mRNA和卵巢中FSHR、LHR mRNA表达量均极显著下降(P＜0.01).结果表明,小花棘豆毒性成分可通过丘脑—垂体—性腺轴影响和田羊母羊的生殖系统.%The study was aimed to research the effects of Oxytropis glabra DC (O.glabra DC)poisoning on reproductive organs coefficent,reproductive performance and related gene expression in Hetian sheep ewes.The sera,hypothalamus,pituitary and ovary of Hetian sheep ewes cases poisoning of O.glabra DC were collected,the organ exponent and contents of GnRH,FSH,LH,E2 and P4 in serum were measured,then the mRNA expression of reproductive genes were measured.The result demonstrated that the index of hypothalamus,pituitary and ovary were extremely significantly higher than normal sheep (P＜0.01),the follicle number on the surface of the ovaries in poisoning groups were significantly lower than normal sheep (P＜0.05).The result of hematoxylin-eosin staining showed that the nuclear of neuronal cells in hypothalamus were pyknosis and hyperchromatic;The nuclear of cells in pituitary deformation and hyperchromatic,cytoplasm decreased;The primary oocyte in ovary dissolved,disappeared,interstitial blood vessel expanded.The content of GnRH,FSH,LH,E2 and P4 in serum in poisoned Hetian sheep ewes were extremely significantly lower than normal sheep (P＜0.01).The mRNA expression levels of Kiss-1,GPR54,ERα in hypothalamus,GnRHR in pituitary and FSHR and LHR in ovary were extremely significantly lower than normal sheep (P＜0.01).The result showed that O.glabra DC poisoning could affect Hetian sheep ewes reproductive system by affecting microstructure and ultrastructure of hypothalamus-pituitary-ovarian axis.