鸡白痢沙门氏菌病原的分离鉴定及耐药性分析

摘要

为鉴定新疆某些地区鸡白痢沙门氏菌主要流行株的流行现状和耐药情况,本研究采用平板凝集试验方法对有发病史的种鸡场进行现场检测,被检的750只鸡中,发现78例疑似鸡白痢沙门氏菌感染,运用沙门氏菌鉴别培养基、组织病理学观察和PCR方法进行确诊,并进行药敏试验.结果显示,疑似的78例鸡白痢沙门氏菌中有69例符合鸡白痢沙门氏菌的生化特性和病理学特点,PCR成功扩增出invA基因,分离率为9.2％,血清型诊断为D1型;其中分离菌对万古霉素和新霉素耐药性最高,耐药率分别为100.0％和81.2％,而对头孢曲松、克拉维酸和多黏菌素的敏感率达60.0％以上.本研究为养殖户和兽医工作者在净化鸡白痢沙门氏菌及临床用药方面提供参考.%In order to investigate the epidemic situation and drug resistance of major epidemic Salmonella Pullorum in some areas of Xinjiang,750 chickens with disease history were detected using the methods of plate agglutination,and a total of 78 cases were diagnosed infection with Salmonella Pullorumin by using Salmonella identification medium,histopathological observation,PCR and drug sensitivity test.The results showed that 69 of 78 cases suspect Salmonella Pullorum were isolated,and prove the biochemical characteristics and pathologic characteristics of Salmonella Pullorum,the invA gene was amplified successfully by PCR,the isolation rate was 9.2 ％ and the serotype was diagnosed as D1.The isolates were the most resistant to vancomycin and neomycin,and Salmonella Pullorum resistant rate was 100.0％ and 81.2％,respectively.Drug sensitive test results showed that the sensitive rate of the bacteria were more than 60.0 ％ to ceftriaxone,clavulanate and polymyxin.This study provided reference for farmers and veterinary workers,which indicated it could be used for the purification of Salmonella Pullorum and clinical medication.