青海海西州地区牛羊布鲁菌氏病监测方案的研究

摘要

目的探索适合青海省海西州地区牛、羊布鲁氏菌病的监测方案,净化布鲁氏菌病.利用琥红平板凝集试验(RBPT)、全乳环状试验(MRT)和试管凝集试验(SAT)对1200份牛血清中376份奶牛血清、677份羊血清,以及376份牛奶进行检测.结果RBPT检测1200份牛血清中9份可疑,3份阳性；376份奶牛血清中3份可疑,1份阳性；其他牛6份可疑,2份阳性；677份羊血清中3份可疑.MRT检测376份奶样中5份可疑,1份阳性.SAT重复检测的20份血清中2份为阳性,RBPT与MRT结果的符合率为100%.结论三种检测方法的联合使用适合大样本的进行布鲁氏菌病的监测,为基层布鲁氏菌病的监测方案的研究提供了理论依据.%　　To explore the suitable cattle and sheep brucellosis surveillance and eradication programs in Haixi Prefecture of Qinghai province region. 1200 bovine serum samples including 376 dairy cow serum samples,677 sheep serum samples and 376 milk samples were detected by using the Rose Bengal plate agglutination test(RBPT),whole milk ring test(MRT)and standard tube agglutination test(SAT).Results showed that among 1200 bovine serum samples,3 were positive and 9 were suspicious(including 1 positive and 3 suspicious among 376 dairy cow serum samples)and among 677 sheep serum samples,3 were suspicious by RBPT.Among 376 milk samples,5 were suspicous by MRT.2 out 20 serum samples retested by SAT were positive.The coincidence between RBPT and MRT for detection of Brucella in serum samples was 100%.In conclusion,joint use of the three assays(RBPT,MRT and SAT)were suitable for brucellosis surveillance of large amount of samples,thus providing a theoretical basis for making grassroots brucellosis surveillance pragrams.