诺沃克病毒实时荧光RT-PCR检测方法的建立

摘要

In this paper,a real-time RT-PCR was developed for detection of Norwalk virus,with the primers and fluorescence probe designed according to the reported genome sequence of Norwalk virus. By the Real-time RT-PCR detection,a specific fluorescence curve was got. It was shown that the test results were exacted and reliable.%　　本文建立了一种用于诺沃克病毒实时荧光RT-PCR检测的引物和方法。分析已报道的诺沃克病毒基因组序列，分别设计引物和荧光探针。通过实时荧光RT-PCR检测，得到诺沃克的特异性荧光曲线，检测结果准确、可靠。