DAPI检测卵泡期绵羊生殖器官中细胞凋亡的比较研究

摘要

为探讨在卵泡期绵羊生殖器官中细胞凋亡的作用机制,本试验以小尾寒羊和萨福克羊为研究对象,采用DAPI(4',6'联脒-2-苯基吲哚)原住荧光标记技术,对高繁殖力品种小尾寒羊和低繁殖力品种萨福克羊成年母羊卵泡期主要生殖器官(卵巢、输卵管、子宫)进行细胞凋亡的确认与定位.结果显示:小尾寒羊和萨福克羊卵巢中卵泡上皮细胞、颗粒细胞、膜细胞均有凋亡现象.在三级卵泡和成熟卵泡的颗粒细胞和膜细胞中,萨福克羊比小尾寒羊表达出较多数量的凋亡细胞,两品种羊在卵泡期输卵管各部,子宫内膜腺体中几乎均未检测到凋亡细胞,子宫内膜基质中检测到少量细胞发生凋亡.因此,研究表明细胞凋亡是母羊生殖器官变化的重要调节机制,可能与造成两者繁殖力高低差异有关.%To study the regulatory mechanism on apoptosis in sheep generative organ at follicular phase, in situ fluorescent labeling (4',6'-diamidino-2-phenylindole, DAPI) was used to confirm and locate apoptosis cells in major reproductive organs (ovaries,oviduct and uterus) of Small-tails Han Sheep (SHS) and Suffolk Sheep (SS) ,which have high and low productivity respectively. The finding of in situ fluorescent labeling (DAPI) indicate that, apoptosis might take place in follicular epithelium cells (FeC),granule cells (GrC) and theca cells (ThC) of SHS and SS. Suffolk Sheep had more apoptosis of GrC and ThC in tertiary follicle and graafian follicle (VG) than SHS. Neither SHS nor SS apoptotic cells were detected in oviduct and glandular organ during follicular phase (FP). While a few endometrial stroma cells (StC) with apoptosis were detected. The result showed that apoptosis is an important regulatory mechanism in reproductive organs of ewe,and it might result in difference in production between SHS and SS.