日本落叶松新鲜松针的化学成分及其抗氧化活性研究

摘要

研究了日本落叶松新鲜松针的化学成分及其抗氧化活性.采用Sephadex LH-20柱色谱及薄层色谱等方法进行分离,从其95%乙醇提取物乙酸乙酯溶性部分中分到7种化合物,经波谱分析及理化性质化合物分别鉴定为:儿茶素(1)、表儿茶素(2)、没食子儿茶素(3)、异鼠李素-3-O-葡萄糖苷(4)、紫云英苷(5)、2"-O-鼠李糖牡荆黄苷(6)和cedrusin (7).7种化合物均首次从该植物中分得.经DPPH试验,测定了核酸溶性部分、二氯甲烷溶性部分、乙酸乙酯溶性部分、水溶性部分、粗提物及分得化合物的抗氧化活性.其中乙酸乙酯溶性部分及化合物1～3具有很强的抗氧化活性.%Seven compounds, including three flavan-3-ols, (+)-catechin (1), (-)-epicatechin (2) and (+)-gallocatechin (3), three flavonoid glycosides, isorhamnetin-3-O-β-D-glucopyranoside (4), astragalin (5) and 2"-O-rhmnosylvitexin (6), and one lignan with the trivial name cedrusin (7), were isolated for the first time from the fresh needles of Larix kaempferi C. Their structures were elucidated by chemical and spectral analysis. The antioxidant activities of the crude extracts, partitioned fractions and isolated compounds were evaluated by DPPH free radicalscavenging assay. Results suggested that EtOAc participated fraction and the three flavan-3-ols showed significantly high antioxidant potential compared with α-tocopherol and BHT, which were used as controls.