杜仲叶提取物的抑菌活性研究

摘要

为了发现新的抑茵材料,对杜仲叶70％(体积分数)乙醇提取物及其分级组分进行了抑菌试验.结果显示:杜仲叶的乙醇和乙酸乙酯提取物对细菌和真茵都有抑制作用,而且乙酸乙酯提取物对黄曲霉和黑曲霉有特殊的抑制特征;正丁醇和水提取物对细菌有抑制作用,但对真菌几乎没有抑制作用;石油醚提取物几乎对细菌和真茵都没有抑制作用.最大抑茵圈直径超过了10 mm,最小抑茵浓度(MIC)在0.25％～3.0％之间.杜仲叶提取物在抑菌活性方面具有一定的热稳定性,抑茵率随时间而逐渐增加,16 h之后基本保持不变.%In order to find new antimicrobial substance,antimicrobial activities of 70%(volume fraction)ethanol extract of Eucommia ulmoides Oliv.leaves and its fractionated extracts were tested.The result showed that ethanol extract and ethyl acetate extract had inhibitory effect toward bacteria and fungi,while ethyl acetate extract had particular inhibition behavior toward A.flauus and A.niger.Butylalcohol extract and aqueous extract had inhibitory effect toward bacteria,but no had inhibitory effect toward fungi.Petroleum ether extract had scarcely inhibitory effect toward bacteria and fungi.The maximal inhibition diameter was above 10 mm,the minimum inhibition concentration was between 0.25%-3.0%.The extracts of E.ulmoides leaves had good thermostability on antimicrobial activities.Inhibition rate gradually increased with time,but hardly changed after 16 h.