松树皮多酚的亚临界水提取及抗氧化活性初探

摘要

Subcritical water was used to extract polyphenol from pine bark. Its effects on the polyphenol yield were investigated. The DPPH radical scavenging activity of polyphenol by subcritical water extraction was determined. The results showed that the optimum process conditions were extraction temperature 150 X., extraction time 5 min, liquid-feed ratio 20: 1 , and extraction pressure 4 Mpa. Under this condition the yield of polyphenol was 4. 86 %. Compared with the ultrasound-assisted extraction and heat reflux extraction, the subcritical water extraction improved the yields of polyphenol by 33. 52 % and 37. 68 % , respectively. The polyphenol extracted by subcritical water had a better DPPH radicals scavenging activity than that of the antioxidant BHA (7. 82 mg/L). The half inhibitory concentration ( IC50 ) of the polyphenol was 7. 10 mg/L.%采用亚临界水从松树皮中提取多酚,考察了反应条件对多酚得率的影响,并与超声波辅助提取法和热回流提取法进行了比较,同时测定了亚临界水提取所得松树皮提取物的DPPH自由基清除能力.结果表明:亚临界水提取松树皮多酚的最佳工艺条件为:提取温度150℃、提取时间5min、液料比20∶ 1(mL∶g)、提取压力4MPa,多酚的提取得率为4.86％,1次提取且相同液料比条件下,比超声波辅助提取法和热回流提取法分别提高了33.52％和37.68％.亚临界水提取所得松树皮提取物对DPPH自由基具有良好的清除效果,其半数抑制质量浓度(IC50)为7.10 mg/L,抗氧化性高于常用抗氧化剂BHA(7.82 mg/L).