在测定不动杆菌(Acinetobacter sp.)Asp16菌株链霉素最低杀菌浓度的基础上,对菌株进行UV和60Co诱变处理后,利用链霉素抗性平板筛选获得1株遗传性能稳定、高转化甘油产1,3-二羟基丙酮的突变株Asr168,将突变株在5.0g·L-1酵母膏、140.0g·L-1甘油、7.0 g·L-1 CaCO3组成的发酵培养基中于30℃、250 r·min-1摇床培养50 h,1,3-二羟基丙酮的产量达到136 g·L-1,比出发菌株提高200％.表明通过UV和60Co诱变,结合链霉素抗性平板筛选,可大幅提高不动杆菌转化甘油产1,3-二羟基丙酮的能力.%Based on determination of Acinetobacter sp. Asp 16 streptomycin minimal bactericidal concentrations mutant strain Asrl68 with high-production of dihydroxyacetone (DHA) and stable genetic performance was screened by UV and 60Co irradiation combining drug-resistance technique. The production of DHA reached 136 g ? L-1 , increasing 200% compared with that of start strain when the mutant strain Asrl68 was cultured at 30 °C ,250 r ? Min~' for 50 h in the optimal fermentation medium as follows: yeast extract 5. 0 g ? L-1,glycerol 140. 0 g ? L-1 ,CaCO3 7. 0 g ? L-1. This screening method can significantly improve the conversion ability of Acinetobacter sp. To produce DHA from glycerol.
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