利用响应面法优化龙葵中澳洲茄边碱的提取工艺。采用单因素实验结合 Box-Behnken 中心组合设计，以澳洲茄边碱提取率为指标，考察乙醇体积分数、提取时间、料液比等因素对提取效果的影响。确定龙葵中澳洲茄边碱的最优提取工艺为：乙醇体积分数80％、提取时间2.1 h、料液比1∶12（g∶mL）、提取2次，在此条件下，澳洲茄边碱提取率为0.721 mg·g－1，与理论值（0.712 mg·g－1）相符，表明工艺可行，预测性较好。从龙葵中提取的澳洲茄边碱能明显抑制 K562、HL-60、BGC-823、MCF-7、Hela 等肿瘤细胞的增殖，具有较强的体外抗肿瘤活性。%Response surface methodology(RSM)was employed to optimize the extraction process of solama-rgine from Solanum nigrum L..Using extraction rate of solamargine as index,the effects of ethanol volume fraction,extraction time and solid-liquid ratio on extraction efficiency were investigated by single factor experi-ment and Box-Behnken central composite design.The optimum extraction conditions were obtained as follows:ethanol volume fraction 80%,extraction time 2.1 h,solid-liquid ratio 1 ∶ 12(g∶mL),extracting for 2 times. Under above conditions,the extraction rate was 0.721 mg· g-1 ,which was well met with the predictive of 0.712 mg·g-1 .Results showed that the process was feasible and had good predictability.Solamargine extracted from Solanum nigrum L.had obvious inhibitory effects on K562,HL-60,BGC-823,MCF-7 and Hela cell lines, and exhibited significant antitumor activities in vitro .
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