甲醛在有无苯作用下对细胞因子M-CSF、G-CSF 和 EPO 转录的影响

摘要

研究了甲醛在有无苯作用下对3种细胞因子巨噬细胞集落刺激因子(M-CSF)、粒细胞集落刺激因子(G-CSF)和促红细胞生成素(EPO)转录水平的影响。将30只雄性 BALA/c 小鼠随机分成5组,设为空白对照组、玉米油对照组、苯染毒组、甲醛染毒组、苯与甲醛联合染毒组,每组6只,分别进行玉米油溶液、苯溶液灌胃染毒或甲醛气态吸入染毒；染毒时间模拟工厂工作时间,每天8 h,连续两周,第6 d、第7 d 休息,第13 d 染毒结束；取小鼠的骨髓和肾脏,提取其总 RNA,并用实时荧光定量 PCR 技术对细胞因子 M-CSF、G-CSF 和 EPO 转录水平的变化进行检测。结果表明,甲醛单独作用可使 M-CSF、G-CSF、EPO 的转录水平下降；与苯联合后,苯加强了甲醛对 M-CSF 转录水平的影响、削弱了甲醛对 G-CSF 和 EPO 转录水平的影响。%Effects of formaldehyde on the transcription level of macrophage colony stimulating factor(M-CSF),granulocyte colony stimulating factor(G-CSF)and erythropoietin(EPO)in the presence and absence of benzene were investigated.Thirty BALA/c male mice were randomly divided into 5 groups(6 for each group):blank control group,corn oil control group,benzene exposure group,formaldehyde exposure group,benzene as-sociated with formaldehyde exposure group.And the mice were exposed by intragastric administration of corn oil solution or benzene solution,formaldehyde gas suction,respectively.Exposure time was simulated factory work schedule,8 h a day for two weeks,with an exposure-free “weekend”on days 6 and 7,sacrificed on day 13. The bone marrow and kidney were prepared to extract total RNA,then real-time fluorescence quantitative PCR technique was applied to test the change of transcription level for cytokine M-CSF,G-CSF and EPO.Results showed that,formaldehyde alone could decrease the transcription level of M-CSF,G-CSF and EPO,when form-aldehyde associated with benzene,benzene could enhance the influence of formaldehyde on transcription level of M-CSF,weaken the influence of formaldehyde on transcription level of G-CSF and EPO.