以菌丝球数量、菌丝球大小、菌丝体生物量及胞内多糖含量为评价指标,筛选香菇发酵的液体种子培养基和发酵培养基,考察了不同补糖方式对香菇摇瓶补料分批发酵的影响.结果表明,适合香菇发酵的液体种子培养基和发酵培养基(g·L-1)为:葡萄糖10,酵母浸粉20,玉米粉15,磷酸二氢钾0.3,硫酸镁0.15,维生素B10.02.在香菇摇瓶补料分批发酵的第6 d和第9 d分别补加0.25%葡萄糖,香菇菌丝体生物量达到约847 mg·(100 mL)-1,胞内多糖含量达到约173 mg·(100 mL)-1,较不加糖时分别增加了约21.79%和34.29%.补料分批发酵能够提高香菇菌丝体生物量和胞内多糖含量,为香菇多糖大规模工业化生产奠定了基础.%Using mycelium pellet number,mycelium pellet size,mycelium biomass,and intracellular polysac-charide content as evaluation indexes,we screened the liquid seed and fermentation media for the fermentation of Lentinus edodes,and investigated the effect of different glucose-feeding modes on the fed-batch fermentation of Lentinus edodes in shake flask.Results showed that the optimal liquid seed and fermentation media were as follows(g·L-1):glucose 10,yeast extract 20,corn flour 15,KH2PO40.3,MgSO40.15,and VB10.02.When 0.25% of glucose was fed at the 6th day and 9th day in the process of fed-batch fermentation of Lentinus edodes in shake flash,the mycelium biomass reached about 847 mg·(100 mL)-1and the intracellular polysaccharide content reached about 173 mg·(100 mL)-1,which increased by about 21.79% and 34.29%,respectively,com-pared to those by non-glucose-feeding fermentment.Fed-batch fermentation can improve the mycelium biomass and intracellular polysaccharide content,which lays a foundation for large-scale industrial production of lentin-an.
展开▼
