金纳米花-人血清白蛋白对色氨酸对映异构体的手性识别

摘要

将人血清白蛋白(HSA)自组装于金纳米花(AuNFs)修饰的玻碳电极表面，从而构建了一个简单的手性传感界面，利用方波伏安技术(SWV)研究了该修饰电极与色氨酸对映异构体(D-Trp和L-Trp)的相互作用。实验结果表明，当D-或L-Trp与手性界面作用后,电极表面电子传递受阻，表现为不同的方波响应；其中L-Trp的峰电流明显低于D-Trp，峰电流差值达到123．7μA；峰电流与浓度在1．0×10-6 mol/L到5．0×10-3 mol/L范围内呈线性相关。%A simple chiral sensing platform for enantioselective recognition of the tryptophan enantiomers was fabricated via the self-assembled of human serum albumin (HSA) on the gold nanoflowers (AuNFs) modified glassy carbon electrode. And the interaction between the modified electrodes and the tryptophan enantiomers (D-Trp and L-Trp) was studied via square wave voltammetry (SWV) . The difference of peak currents in SWV display that the transmission of the electron on the electrode surface can be hindered by the interaction between D-or L-Trp and the chiral interface. The peak currents of L-Trp were significantly lower than that of D-Trp and the difference of peak currents reached 123.7μA. The peak currents of L-Trp or D-Trp were linearly related to the concentration in the range of 1.0 × 10-6 mol/L to 5.0 × 10-3 mol/L.