Chiral recognition of penicillamine enantiomers using hemoglobin and gold nanoparticles functionalized graphite-like carbon nitride nanosheets via electrochemiluminescence

摘要

A new stable and stereo-selective electrochemiluminescence (ECL) interface has been designed for specific recognition of penicillamine (Pen) enantiomers by using hemoglobin (Hb) and gold nanoparticles functionalized graphite-like carbon nitride nanosheets composite (Au-g-C3N4 NHs) modified glassy carbon electrodes (Hb/Au-g-C3N4/GCE). The advantages of Hb as chiral selector and Au-g-C3N4 NHs as luminophore were perfectly displayed in this novel interface. The obviously different ECL intensity was exhibited after L-Pen and n-Pen adsorbed on HbfAu-g-C3N4/GCE, and a larger response was observed on D-Pen/Hb/Au-g-C3N4/GCE. Under the optimum conditions, the developed ECL chiral sensor showed excellent analytical property for detection of Pen enantiomers in a linear range of 1.0 x 10(-4) M to 5.0 x 10(-3) M, and the detection limits L-Pen and D-Pen were 3.1 x 10(-5) M and 3.3 x 10(-5) M (S/N = 3) respectively. This work with high selectivity, stability and reproducibility may open a new door based on ECL to discriminate Pen enantiomers. (C) 2016 Elsevier B.V. All rights reserved.