以甲基化-β-环糊精为功能单体制备的L-色氨酸印迹聚合物及其性能研究

摘要

以 L-色氨酸( L-Trp)为模板分子,甲基化-β-环糊精( M-β-CD)为功能单体,优化印迹体系和聚合方法制备了12种印迹聚合物MIP1~12,采用SEM和N2吸附试验表征优化印迹聚合物的结构,通过吸附动力学曲线和吸附等温线模型探讨其吸附特性,并结合HPLC分析聚合物对DL-Trp的手性拆分能力。结果表明：以纳米二氧化硅( nano-SiO2)为载体,采用表面聚合法制备的印迹聚合物MIP12具有最优的印迹效果。 MIP12为孔径分布较为均匀的中孔材料,主要存在两类结合位点,吸附过程符合准二级动力学模型。 MIP12表现出满意的手性拆分能力,对DL-Trp拆分因子为2．784；在由六种芳香族氨基酸构建的复杂体系中,MIP12也能特异性拆分色氨酸,拆分因子为1．830,具有较好的应用前景。%Twelve imprinted polymers were prepared with L-Trp as template,methylated-β-cyclodextrin( M-β-CD) as functional mon-omer by varying the imprinting components and polymerization process. The optimum imprinting polymer( MIP12 ) was characterized in structure,binding characteristics and chiral resolution ability. The obtained data revealed a mesoporous material with two classes of binding sites and pesdo-second dynamics adsorption model for L-Trp. MIP12 showed a desired chiral resolvability for the racemic tryptophan with a resolution factor of 2. 784. Even in the complex system composed by racemic tryptophan,tyrosine and phenylala-nine,MIP12 can recognize L-Trp superior to D-Trp. The results implied a potential application prospect of MIP12.